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Modulation of the resorption and osteoconductivity of α-calcium sulfate by histone deacetylase inhibitors

Paper ID Volume ID Publish Year Pages File Format Full-Text
10020 659 2010 9 PDF Available
Title
Modulation of the resorption and osteoconductivity of α-calcium sulfate by histone deacetylase inhibitors
Abstract

Calcium sulfate (CS) is an osteoconductive material with a long history of clinical use. However, its resorptive properties are not optimal for bone regeneration. Recently, histone deacetylase inhibitors (HDIs) have been suggested as bone regeneration tools. In this study, we investigated the effects of the HDIs sodium butyrate and trichostatin A on α-form CS (αCS) performance. MC3T3-E1 pre-osteoblasts cultured on αCS containing either HDI (αCS/HDI) showed higher levels of alkaline phosphatase activity than those cultured on αCS alone. The expression of genes characteristic of the osteoblast phenotype, including Runx2, osteocalcin, and bone sialoprotein, was strongly promoted by αCS/HDI. When cultured on αCS/HDIs, the osteoclastic differentiation of RAW264.7 monocytes was substantially suppressed, as measured by tartrate-resistant acid phosphatase (TRAP) activity and the expression levels of calcitonin receptor and TRAP. Neither HDI affected the CS setting time, compressive strength, or dissolution in a simulated body fluid. In a rat calvarial model of critical size bone defects, αCS/HDIs enhanced osteoblast differentiation, led to new bone formation, and delayed resorption, as confirmed by micro-computed tomography and histological analyses.

Keywords
Alpha calcium sulfate; Histone deacetylase inhibitors; Bone regeneration; Osteoblasts; Osteoclasts
First Page Preview
Modulation of the resorption and osteoconductivity of α-calcium sulfate by histone deacetylase inhibitors
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 1, January 2010, Pages 29–37
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering