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The role of oxygen termination of nanocrystalline diamond on immobilisation of BMP-2 and subsequent bone formation

Paper ID Volume ID Publish Year Pages File Format Full-Text
10049 661 2008 10 PDF Available
Title
The role of oxygen termination of nanocrystalline diamond on immobilisation of BMP-2 and subsequent bone formation
Abstract

Medical implants are increasingly often inserted into bone of frail patients, who are advanced in years. Due to age, severe trauma or pathology-related bone changes, osseous healing at the implant site is frequently limited. We were able to demonstrate that coating of endosseous implants with nanocrystalline diamond (NCD) allows stable functionalization by means of physisorption with BMP-2. Strong physisorption was shown to be directly related to the unique properties of NCD, and BMP-2 in its active form interacted strongly when NCD was oxygen-terminated. The binding of the protein was monitored under physiological conditions by single molecule force spectroscopy, and the respective adsorption energies were further substantiated by force-field-calculations. Implant surfaces refined in such a manner yielded enhanced osseointegration in vivo, when inserted into sheep calvaria. Our results further suggest that this technical advancement can be readily applied in clinical therapies with regard to bone healing, since primary human mesenchymal stromal cells strongly activated the expression of osteogenic markers when being cultivated on NCD physisorbed with physiological amounts of BMP-2.

Keywords
Nanocrystalline diamond (NCD); Physisorption; Bone morphogenetic protein-2 (BMP-2); Bone regeneration; Mesenchymal stem cells; Nanotopography
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The role of oxygen termination of nanocrystalline diamond on immobilisation of BMP-2 and subsequent bone formation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 16, June 2008, Pages 2433–2442
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us