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The use of chondrogenic differentiation drugs to induce stem cell differentiation using double bead microsphere structure

Paper ID Volume ID Publish Year Pages File Format Full-Text
10054 661 2008 11 PDF Available
Title
The use of chondrogenic differentiation drugs to induce stem cell differentiation using double bead microsphere structure
Abstract

A double bead microsphere device that functions as a 3D scaffold and also delivers bioactive molecules to cells has been developed. The system we developed consists of large polymeric microspheres that were loaded with dexamethasone (DEXA) and coated with DHEA, which were physically immobilized using a layer-by-layer (LbL) system. The initial step in this strategy involves the creation of microparticles that contain DHEA, which were simply produced using a water-in-oil-in-water (W/O/W) emulsion method. In the second step, small sized microparticles containing DHEA were coated onto positively-charged poly(d,l-lactic-co-glycolic acid) (PLGA) microspheres that contained DEXA pretreated with poly(ethyleneimmine) (PEI). Microsphere constructs that contain DEXA and DHEA showed a significantly higher number of specific lacuna phenotypes at the end of a 4-week study in vitro and at the end of a 6-week study in vivo, irrespective of the presence of DEXA and DHEA. Therefore, the dual delivery of DEXA and DHEA can be used to engineer inflammation-free tissue in the vicinity of the implant. These double beaded PLGA microsphere constructs containing DEXA and DHEA show promise as coatings for implantable biomedical devices to improve biocompatibility and ensure in vivo performance.

Keywords
Microsphere; Scaffold; LbL; Cell differentiation; DEXA; DHEA
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The use of chondrogenic differentiation drugs to induce stem cell differentiation using double bead microsphere structure
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 16, June 2008, Pages 2490–2500
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us