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RGD—Functionalized polymer brushes as substrates for the integrin specific adhesion of human umbilical vein endothelial cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
10064 662 2007 11 PDF Available
Title
RGD—Functionalized polymer brushes as substrates for the integrin specific adhesion of human umbilical vein endothelial cells
Abstract

This report demonstrates the feasibility of surface-initiated atom transfer radical polymerization to prepare thin polymer layers (“brushes”) that can be functionalized with short peptide ligands and which may be of use as coatings to promote endothelialization of blood-contacting biomaterials. The brushes are composed of poly(2-hydroxyethyl methacrylate) (PHEMA) or poly(poly(ethylene glycol) methacrylate) (PPEGMA), which do not only suppress non-specific adhesion of proteins and cells but also contain hydroxyl groups that can be used to introduce small peptide ligands. A protocol has been developed that allows functionalization of the brushes with RGD containing peptide ligands resulting in surface concentrations ranging from ∼0.5–12 pmol/cm2. At peptide surface concentrations >1–5.3 pmol/cm2, human umbilical vascular endothelial cells (HUVECs) were found to adhere and spread rapidly. A difference in size and morphology of focal adhesions between HUVECs immobilized on PHEMA and PPEGMA brushes was observed. It is proposed that this is due to the increased ethylene glycol spacer length and hydrophilicity of the PPEGMA brushes, which may lead to increased ligand mobility and reduced ligand–integrin affinity. HUVECs immobilized on the polymer brushes were also found to be able to retain homeostasis when exposed to shear stresses that simulated arterial blood flow.

Keywords
Surface-initiated atom transfer radical polymerization; Polymer brushes; Poly(poly(ethylene glycol) methacrylate); Poly (2-hydroxyethyl methacrylate); RGD peptide; Cell adhesion
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RGD—Functionalized polymer brushes as substrates for the integrin specific adhesion of human umbilical vein endothelial cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 16, June 2007, Pages 2536–2546
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us