fulltext.study @t Gmail

A novel hydrogel-collagen composite improves functionality of an injectable extracellular matrix

Paper ID Volume ID Publish Year Pages File Format Full-Text
1007 71 2011 10 PDF Available
Title
A novel hydrogel-collagen composite improves functionality of an injectable extracellular matrix
Abstract

Cellular transplantation is now closer to becoming a practical clinical strategy to repair, regenerate or restore the function of skin, muscle, nerves and pancreatic islets. In this study we sought to develop a simple injectable collagen matrix that would preserve the normal cellular organization of skin cells. Three different scaffolds were created and compared: collagen–glycosaminoglycan (GAG) scaffolds, crosslinked collagen–GAG scaffolds without polyvinyl alcohol (PVA) and crosslinked collagen–GAG scaffolds containing PVA hydrogel. Importantly, all scaffolds were found to be non-cytotoxic. PVA-containing gels exhibited a higher tensile strength (P < 0.05), faster fibril formation (P < 0.001) and reduced collagenase digestion (P < 0.01) compared with other gels. Free floating fibroblast-populated, PVA–borate scaffolds resisted contraction over a 10 day period (P < 0.001). The fibroblast-populated scaffolds containing PVA demonstrated a 3-fold reduction in cellularity over 10 days compared with the control gels (P < 0.001). Multicellular skin substitutes containing PVA–borate networks display a linear cellular organization, reduced cellularity and the formation of a keratinized epidermis that resembles normal skin. In conclusion, these data underscore the multifunctionality of a simple PVA–borate–collagen matrix as an injectable composite for tissue engineering or cell transplantation.

Keywords
Injectable scaffold; Collagen; Polyvinyl alcohol; Extracellular matrix; Skin substitute
First Page Preview
A novel hydrogel-collagen composite improves functionality of an injectable extracellular matrix
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 8, August 2011, Pages 3060–3069
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us