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Highly biocompatible multi-walled carbon nanotube–chitosan nanoparticle hybrids as protein carriers

Paper ID Volume ID Publish Year Pages File Format Full-Text
1008 71 2011 8 PDF Available
Title
Highly biocompatible multi-walled carbon nanotube–chitosan nanoparticle hybrids as protein carriers
Abstract

Carbon nanotube (CNT)–organic polymer hybrids have important potential applications in the immobilization of therapeutic biomolecules. Recently developed CNT–organic polymer composites require the use of organic solvents for their preparation and have limited polymer functionalization. To address these limitations, multi-walled CNT (MWCNT)–chitosan nanoparticle (CS NP) hybrids have been synthesized in situ by an ionotropic gelation process, which is extremely mild and involves the mixture of two aqueous solutions at room temperature. The MWCNT–CS NP hybrids were characterized by atomic force microscopy and thermogravimetric analysis. Under optimal conditions the CS NP can be tethered to the MWCNT surface in high density and with relatively uniform coverage. The MWCNT–CS NP hybrids show good dispersibility and stability in aqueous solutions. In order to evaluate the potential utilization of the hybrids as protein carries the cytotoxicity to HeLa cells and protein immobilization (of bovine serum albumin (BSA), used here as a model) capacity of the hybrids were investigated in detail. The results demonstrate that the MWCNT–CS NP hybrids are biocompatible at concentrations up to 100 μg mL−1 for 24 h incubation. The MWCNT–CS NP hybrids improve the BSA immobilization efficiency 0.8 times and simultaneously decrease the cellular toxicity by about 50% compared with carboxylated MWCNT.

Keywords
Multi-walled carbon nanotube–chitosan nanoparticle hybrids; In situ synthesis; Ionotropic gelation; Cytotoxicity; Protein immobilization capacity
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Highly biocompatible multi-walled carbon nanotube–chitosan nanoparticle hybrids as protein carriers
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 8, August 2011, Pages 3070–3077
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us