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A hairpin aptamer-based electrochemical biosensing platform for the sensitive detection of proteins

Paper ID Volume ID Publish Year Pages File Format Full-Text
10089 663 2009 6 PDF Available
Title
A hairpin aptamer-based electrochemical biosensing platform for the sensitive detection of proteins
Abstract

An aptamer-based electrochemical sensing platform for the direct protein detection has been developed using IgE and a specifically designed aptamer with hairpin structure as the model analyte and probe sequence, respectively. In the absence of IgE, the aptamer immobilized on an electrode surface forms a large hairpin due to the hybridization of the two complementary arm sequences, and peak currents of redox species dissolved in solution can be achieved. However, the target protein binding can not only cause the increase of the dielectric layer but also trigger the significant conformational switching of the aptamer due to the opening of the designed hairpin structure that pushes the biomolecule layer/electrolyte interface away from the electrode surface, suppressing substantially the electron transfer (eT) and resulting in a strong detection signal. The detection limit of 3.6 × 10−11 M and linear response range of 5.4 × 10−11 to 3.6 × 10−8 M are achieved without any amplifier. The selectivity is confirmed by interference test. More importantly, an innovative concept of adapting intelligently a surface-confined aptamer sequence is introduced, and the limitations of the conventional electrochemical aptasensors have been overcome. The proposed sensing scheme is expected to become a promising strategy for the detection of proteins and other biomacromolecules.

Keywords
Aptamer probe; Conformational change; Electron-transfer distance; Alternating current voltammetry
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A hairpin aptamer-based electrochemical biosensing platform for the sensitive detection of proteins
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 15, May 2009, Pages 2950–2955
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us