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The characteristics and transfection efficiency of cationic poly (ester–co–urethane) – short chain PEI conjugates self-assembled with DNA

Paper ID Volume ID Publish Year Pages File Format Full-Text
10103 664 2009 9 PDF Available
Title
The characteristics and transfection efficiency of cationic poly (ester–co–urethane) – short chain PEI conjugates self-assembled with DNA
Abstract

To improve the transfection efficiency of polycations with DNA, we synthesized poly(ester–co–urethane)(PEU-g-PEI800) with short chain PEI800 in the side chain, and poly(ester–co–urethane)(PEU) without short chain PEI800. Both PEU-g-PEI800 and PEU, readily self-assembled with plasmid DNA (pCMV-βgal) in a HEPES buffer, were characterized by dynamic light scattering and zeta-potential. The results reveal that PEU-g-PEI800 and PEU were able to self-assemble particles with DNA and yield nano-sized complexes (<200 nm) with positive charge at N/P ratios of 20/1 and 120/1, respectively. The degradation studies indicate that the half-life of PEU-g-PEI800 and PEU in the HEPES buffer were 14 and 35 h at pH 7.4, respectively. Titration studies were performed to determine the buffering capacities of the polymers. The COS-7 cell viabilities in the presence of PEU-g-PEI800/DNA, PEU/DNA, and PEI25k/DNA were studied. In addition, The PEU-g-PEI800/DNA complexes were able to transfect COS-7 cells in vitro with a high efficiency comparable to a well-known gene carrier PEI25k. The results indicate that PEU-g-PEI800 is an attractive cationic poly (ester–co–urethane) for gene delivery and an interesting candidate for further study.

Keywords
Transfection; poly(ester–co–urethane); Poly(ethylenimine); Buffering capacity
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The characteristics and transfection efficiency of cationic poly (ester–co–urethane) – short chain PEI conjugates self-assembled with DNA
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 34, December 2009, Pages 6665–6673
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us