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On the effect of substrate curvature on cell mechanics

Paper ID Volume ID Publish Year Pages File Format Full-Text
10104 664 2009 13 PDF Available
Title
On the effect of substrate curvature on cell mechanics
Abstract

Cell movement on a substrate or within the extracellular matrix is the phenomenological response to a biochemical signals' cascade transcripted into biophysical processes and viceversa. The process is complex in nature, including different length scales from the whole cell to organelle and protein levels, where substrate/ECM curvature has been shown to play an important role on cell's behavior. From a macroscopic perspective, the cytoskeleton may be modeled as a continuum body unbalanced by internal protein motors. In this work, we propose a cell constitutive model to simulate cell attachment on curved substrates, activated by contractile forces. We first analyze a single fiber bundle composed by microtubules, actin filaments and myosin machinery. Then, the model is macroscopically extended to the cytoskeletal level using homogenization. Substrate curvature has two implications in our model: (i) it forces fibers to work in a curved (bent) position and (ii) it eventually creates a pre-deformation state in the cytoskeleton. Interestingly, the model shows higher contractile force inhibition as curvature increases when implemented over different substrate morphologies, being this consistent with experimental results. The presented model may result useful in many new regenerative medicine techniques, miniaturized experimental tests, or to analyze cell behavior on manufactured nanoscaffolds for tissue engineering.

Keywords
Cell mechanics; Substrate curvature; Cell–matrix interaction; Computational model; Mechanobiology
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On the effect of substrate curvature on cell mechanics
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 34, December 2009, Pages 6674–6686
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us