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SPR microscopy and its applications to high-throughput analyses of biomolecular binding events and their kinetics

Paper ID Volume ID Publish Year Pages File Format Full-Text
10134 667 2007 13 PDF Available
Title
SPR microscopy and its applications to high-throughput analyses of biomolecular binding events and their kinetics
Abstract

Surface plasmon resonance (SPR) sensing has long been used to study biomolecular binding events and their kinetics in a label-free way. This approach has recently been extended to SPR microscopy, which is an ideal tool for probing large microarrays of biomolecules for their binding interactions with various partners and the kinetics of such binding. Commercial SPR microscopes now make it possible to simultaneously monitor binding kinetics on >1300 spots within a protein microarray with a detection limit of ∼0.3 ng/cm2, or <50 fg per spot (<1 million protein molecules) with a time resolution of 1 s, and spot-to-spot reproducibility within a few percent. Such instruments should be capable of high-throughput kinetic studies of the binding of small (∼200 Da) ligands onto large protein microarrays. The method is label free and uses orders of magnitude less of the precious biomolecules than standard SPR sensing. It also gives the absolute bound amount and binding stoichiometry.

Keywords
Surface plasmon resonance microscopy; Surface plasmon resonance imaging; Bioaffinity; Kinetics; Protein arrays; DNA arrays
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SPR microscopy and its applications to high-throughput analyses of biomolecular binding events and their kinetics
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 15, May 2007, Pages 2380–2392
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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