fulltext.study @t Gmail

Probing molecular recognition sites on biosurfaces using AFM

Paper ID Volume ID Publish Year Pages File Format Full-Text
10135 667 2007 10 PDF Available
Title
Probing molecular recognition sites on biosurfaces using AFM
Abstract

Knowledge of the molecular forces that drive receptor–ligand interactions is a key to gain a detailed understanding of cell adhesion events and to develop novel applications in biomaterials science. Until recently, there was no tool available for analyzing and mapping these forces on complex biosurfaces like cell surfaces. During the past decade, however, single-molecule atomic force microscopy (AFM) has opened exciting new opportunities for detecting and localizing molecular recognition forces on artificial biosurfaces and on living cells. In this review, we describe the general principles of the AFM technique, present procedures commonly used to prepare samples and tips, and discuss a number of applications that are relevant to the field of biomaterials.

Keywords
Atomic force microscopy; Biosurfaces; Molecular recognition; Single molecules; Functionalized tips; Receptor–ligand interactions
First Page Preview
Probing molecular recognition sites on biosurfaces using AFM
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 15, May 2007, Pages 2393–2402
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering