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Dynamic force microscopy imaging of plasmid DNA and viral RNA

Paper ID Volume ID Publish Year Pages File Format Full-Text
10136 667 2007 9 PDF Available
Title
Dynamic force microscopy imaging of plasmid DNA and viral RNA
Abstract

Plasmid DNA and viral RNA were imaged in a liquid environment by dynamic force microscopy (DFM) and fine structures of DNA with heights of 1.82±0.66 nm were obtained in topographical images. In simultaneously acquired phase images, DNA could be imaged with better contrast at lower imaging forces. By splitting the cantilever oscillation signal into lower and upper parts, the contribution of the adhesion between tip and sample to the topographical images was eliminated, resulting in better signal-to-noise ratio. DFM of the single stranded RNA genome of a human rhinovirus showed loops protruding from a condensed RNA core, 20–50 nm in height. The mechanical rigidity of the RNA was determined by single molecule pulling experiments. From fitting RNA stretching curves to the Worm-Like-Chain (WLC) model a persistence length of 1.0±0.17 nm was obtained.

Keywords
Atomic force microscope; Dynamic force microscopy; DNA; RNA; Imaging
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Dynamic force microscopy imaging of plasmid DNA and viral RNA
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 15, May 2007, Pages 2403–2411
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us