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The influence of Ni(II) on surface antigen expression in murine macrophages

Paper ID Volume ID Publish Year Pages File Format Full-Text
10152 668 2009 10 PDF Available
Title
The influence of Ni(II) on surface antigen expression in murine macrophages
Abstract

Biomedical alloys may release nickel ions during corrosion phenomena and, in addition to their interaction with oral tissues, these ions may also influence characteristic properties of the immune system cells. The aim of this study was to evaluate the effect of nickel chloride on the expression of functionally distinct surface antigens in murine RAW macrophages. The expression of the surface antigens CD14, CD40, MHC class I, MHC class II, CD80, CD86, CD54 was analyzed by flow cytometry. The bacterial endotoxin lipopolysaccharide (LPS) was used as a positive control to induce antigen expression. Cells were stimulated with NiCl2 (0.1 and 0.5 mm) in the presence and absence of LPS (0.1 or 25 μg/ml). After exposure periods of 6, 24 and 48 h, LPS caused a time- and dose-dependent increase in the expression of all surface antigens. CD14 expression was up-regulated by 0.1 μg/ml LPS by about 10-fold after 24 h and 100-fold after 48 h. After 48 h, NiCl2 alone up-regulated the expression of all surface antigens between 2- and 4-fold, while in cells stimulated by LPS, 0.1 mm NiCl2 was effective only on CD14, CD40 and MHC class I. Moreover, 0.5 mm NiCl2 even inhibited the LPS-induced expression of all surface antigens, except for CD54, which was still significantly up-regulated. These results show that nickel chloride is able to induce an up-regulation of surface antigen expression, but a high concentration may impair essential functions of macrophages stimulated by LPS.

Keywords
Nickel; Biocompatibility; Macrophages; Surface antigens
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The influence of Ni(II) on surface antigen expression in murine macrophages
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 8, March 2009, Pages 1492–1501
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us