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Akt1 silencing efficiencies in lung cancer cells by sh/si/ssiRNA transfection using a reductable polyspermine carrier

Paper ID Volume ID Publish Year Pages File Format Full-Text
10167 668 2009 13 PDF Available
Title
Akt1 silencing efficiencies in lung cancer cells by sh/si/ssiRNA transfection using a reductable polyspermine carrier
Abstract

Efforts directed in ameliorating silencing studies with shRNA, siRNA and ssiRNA (siRNA with sticky overhangs) are faltered mainly due to the lack of efficient carrier system. In the present study, we developed reductable polyspermine (RPS) carrier composed of multiple spermine units with disulfide linkages for gene expression and silencing studies. In gene expression studies, EGFP expression was found to be almost 4 folds higher and 20 folds safer with RPS carrier than with PEI25K. Moreover, on systemic administration, RPS exhibited significantly high EGFP expression in mice lungs. Similarly in gene silencing studies, EGFP silencing achieved was nearly 1.5 times superior with RPS carrier than PEI25K. Also, RPS delivered Akt1 shRNA (shAkt), siRNA (siAkt) and ssiRNA (ssiAkt) efficiently silenced oncoprotein Akt1 and thereby decreased A549 cell survival. The degrees of cell survival, proliferation and metastasis were differed with the nature of siRNA treatment. Further study at different time intervals revealed that ssiAkt treatment, although superior to sh/siAkt, was highly transient while, shAkt treatment was uniform and prolong. These finding demonstrate the potential use of RPS carrier in gene expression and silencing studies, and significance of the nature of siRNA employed in cancer study.

Keywords
Spermine; siRNA delivery; Reductable carrier; Gene delivery; Akt; siRNA comparison
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Akt1 silencing efficiencies in lung cancer cells by sh/si/ssiRNA transfection using a reductable polyspermine carrier
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 8, March 2009, Pages 1635–1647
Authors
, , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us