fulltext.study @t Gmail

Heparin intercalation into reconstituted collagen I fibrils: Impact on growth kinetics and morphology

Paper ID Volume ID Publish Year Pages File Format Full-Text
10181 670 2008 14 PDF Available
Title
Heparin intercalation into reconstituted collagen I fibrils: Impact on growth kinetics and morphology
Abstract

Collagen type I fibrils, reconstituted in vitro in the presence of heparin, exhibit an unusually thick and straight shape. A detailed structural analysis by scanning force and scanning electron microscopy revealed a non-linear dependence in size distribution, width-to-length ratio, and morphology over a wide range of glycosaminoglycan (GAG) concentrations. By varying molecular weight, degree of sulphation, charge, and concentration of different GAGs we are able to correlate the morphological data with kinetic turbidimetric measurements, and quantitation of fibril-bound GAG. The experiments imply a pronounced impact of the prenucleation phase on the cofibril morphology as a result of the strong electrostatic interaction of heparin with tropocollagen. Heparin is assumed to stabilize the collagen microfibrils and to enhance their parallel accretion during cofibrillogenesis with preservation of the typical asymmetric collagen banding pattern. The heparin quantitation data show heparin to be intercalated as a linker molecule with one specific binding site inside the cofibrils. The reconstituted cofibrils with their unusual morphology and GAG intercalation—a phenomenon not reported in vivo—can be expected to exhibit interesting mechanical and biochemical behaviours as a biomaterial for extracellular matrix scaffolds.

Keywords
Collagen; Heparin; Glycosaminoglycan; Molecular weight; ECM; Charge
First Page Preview
Heparin intercalation into reconstituted collagen I fibrils: Impact on growth kinetics and morphology
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 1, January 2008, Pages 1–14
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us