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The effect of conjugation to gold nanoparticles on the ability of low molecular weight chitosan to transfer DNA vaccine

Paper ID Volume ID Publish Year Pages File Format Full-Text
10192 670 2008 7 PDF Available
Title
The effect of conjugation to gold nanoparticles on the ability of low molecular weight chitosan to transfer DNA vaccine
Abstract

Nonviral gene delivery systems based on conventional high molecular weight chitosans are efficient as DNA vaccine delivery system, but have poor physical properties such as aggregated shapes, low solubility at neutral pH, high viscosity at concentrations used for in vivo delivery and a slow onset of action. Furthermore, Chitosan oligomers shorter than 14 monomers units were recently found to form only weak complexes with DNA, resulting in physically unstable polyplexes in vitro and in vivo. Here, low molecular weight chitosans with an average molecular mass of 6 kDa (Chito6) have been covalently attached to gold nanoparticles (GNPs), and the potency of the resulting Chito6-GNPs conjugates as vectors for the delivery of plasmid DNA has been investigated in vitro and in vivo. After delivery by intramuscular immunization in BALB/c mice, the Chito6-GNPs conjugates induced an enhanced serum antibody response 10 times more potent than naked DNA vaccine. Additionally, in contrast to naked DNA, the Chito6-GNPs conjugates induced potent cytotoxic T lymphocyte responses at a low dose.

Keywords
Chitosan modified gold nanoparticle; DNA vaccination; Gene delivery; Immunogenicity; Nanocarriers
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The effect of conjugation to gold nanoparticles on the ability of low molecular weight chitosan to transfer DNA vaccine
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 1, January 2008, Pages 111–117
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us