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The use of biodegradable polymeric nanoparticles in combination with a low-pressure gene gun for transdermal DNA delivery

Paper ID Volume ID Publish Year Pages File Format Full-Text
10207 671 2008 10 PDF Available
Title
The use of biodegradable polymeric nanoparticles in combination with a low-pressure gene gun for transdermal DNA delivery
Abstract

Gold particles have been used as a carrier for transdermal gene delivery, which may cause adverse side effects when accumulated. In this study, biodegradable nanoparticles, composed of chitosan (CS) and poly-γ-glutamic acid (γ-PGA), were prepared by an ionic-gelation method for transdermal DNA delivery (CS/γ-PGA/DNA) using a low-pressure gene gun. The conventional CS/DNA without the incorporation of γ-PGA was used as a control. Small-angle X-ray scattering (SAXS) was used to examine the internal structures of test nanoparticles, while identification of their constituents was conducted by Fourier transformed infrared (FT-IR) spectroscopy. The CS/γ-PGA/DNA were spherical in shape with a relatively homogeneous size distribution. In contrast, CS/DNA had a heterogeneous size distribution with a donut, rod or pretzel shape. Both test nanoparticles were able to effectively retain the encapsulated DNA and protect it from nuclease degradation. As compared with CS/DNA, CS/γ-PGA/DNA improved their penetration depth into the mouse skin and enhanced gene expression. These observations may be attributed to the fact that CS/γ-PGA/DNA were more compact in their internal structures and had a greater density than their CS/DNA counterparts, thus having a larger momentum to penetrate into the skin barrier. The results revealed that CS/γ-PGA/DNA may substitute gold particles as a DNA carrier for transdermal gene delivery.

Keywords
Transdermal gene delivery; Gene carrier; Degradable nanoparticle; Gene gun
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The use of biodegradable polymeric nanoparticles in combination with a low-pressure gene gun for transdermal DNA delivery
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 6, February 2008, Pages 742–751
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us