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Systematic study of osteoblast and fibroblast response to roughness by means of surface-morphology gradients

Paper ID Volume ID Publish Year Pages File Format Full-Text
10229 674 2007 8 PDF Available
Title
Systematic study of osteoblast and fibroblast response to roughness by means of surface-morphology gradients
Abstract

The surface roughness of a medical implant is of great importance since the surface is in direct contact with the host tissue (e.g. bone, fibrous tissue). The response of cells to roughness is different depending on the cell type. However, the influence of roughness on cell behavior has only rarely been systematically studied. We have developed a surface-modification process to produce roughness gradients that cover a wide range of roughness values on one substratum. Such gradients allow for systematic investigations of roughness on cell behavior. Gradients were fabricated using a two-step roughening and smoothening process, involving sandblasting and a subsequent chemical polishing step. In order to produce a set of identical surfaces we applied a replica technique. Cell experiments were carried out with rat calvarial osteoblasts (RCO) and human gingival fibroblasts (HGF). RCOs showed a significantly increased proliferation rate with increasing surface roughness. The footprint of osteoblasts varied in size at different positions on the gradient, remaining small on the rough end of the gradient and increasing considerably as the roughness decreased. HGF showed the opposite proliferation behavior, proliferation decreasing with increasing roughness. The fibroblast morphology was found to be similar to that seen for osteoblasts.

Keywords
Osteoblast; Fibroblast; Cell proliferation; Cell morphology; Surface roughness; Surface modification
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Systematic study of osteoblast and fibroblast response to roughness by means of surface-morphology gradients
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 13, May 2007, Pages 2175–2182
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us