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Biomechanical and biochemical characteristics of a human fibroblast-produced and remodeled matrix

Paper ID Volume ID Publish Year Pages File Format Full-Text
10230 674 2007 9 PDF Available
Title
Biomechanical and biochemical characteristics of a human fibroblast-produced and remodeled matrix
Abstract

We report on a culture method for the rapid production of a strong and thick natural matrix by human cells for tissue engineering applications. Dermal fibroblasts were cultured for three weeks at high density on porous substrates in serum-containing or chemically defined media. The mechanical and biochemical properties of the resulting cell-derived matrix (CDM) were compared to those of standard fibroblast-populated collagen and fibrin gels and native human skin. We found that the ultimate tensile strength of CDM cultured in our chemically defined media (313±8.7 kPa) is significantly greater than for collagen gels (168±39.3 kPa), fibrin gels (133±8.0 kPa) and CDM cultured with serum (223±9.0 kPa), but less than native skin (713±55.2 kPa). In addition to the biomechanics, this *CDM is also biochemically more similar to native skin than the collagen and fibrin gels in terms of all parameters measured. As *CDM is produced by human cells in a chemically defined culture medium and is mechanically robust, it may be a viable living tissue equivalent for many connective tissue replacement applications requiring initial mechanical stability yet a high degree of biocompatibility.

Keywords
Extracellular matrix (ECM); Soft tissue biomechanics; Mechanical properties; Connective tissue; Wound healing
First Page Preview
Biomechanical and biochemical characteristics of a human fibroblast-produced and remodeled matrix
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 13, May 2007, Pages 2183–2191
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering