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Tetanus toxin C fragment-conjugated nanoparticles for targeted drug delivery to neurons

Paper ID Volume ID Publish Year Pages File Format Full-Text
10260 675 2007 9 PDF Available
Title
Tetanus toxin C fragment-conjugated nanoparticles for targeted drug delivery to neurons
Abstract

The use of nanoparticles for targeted drug delivery is often facilitated by specific conjugation of functional targeting molecules to the nanoparticle surface. We compared different biotin-binding proteins (avidin, streptavidin, or neutravidin) as crosslinkers to conjugate proteins to biodegradable nanoparticles prepared from poly(lactic-co-glycolic acid) (PLGA)–polyethylene glycol (PEG)-biotin polymers. Avidin gave the highest levels of overall protein conjugation, whereas neutravidin minimized protein non-specific binding to the polymer. The tetanus toxin C fragment (TTC), which is efficiently retrogradely transported in neurons and binds to neurons with high specificity and affinity, retained the ability to bind to neuroblastoma cells following amine group modifications. TTC was conjugated to nanoparticles using neutravidin, and the resulting nanoparticles were shown to selectively target neuroblastoma cells in vitro. TTC-conjugated nanoparticles have the potential to serve as drug delivery vehicles targeted to the central nervous system.

Keywords
Nanoparticle; Drug delivery; Brain; Cell culture; Nerve; Protein
First Page Preview
Tetanus toxin C fragment-conjugated nanoparticles for targeted drug delivery to neurons
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 34, December 2007, Pages 5176–5184
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering