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The effect of enzymatically degradable poly(ethylene glycol) hydrogels on smooth muscle cell phenotype

Paper ID Volume ID Publish Year Pages File Format Full-Text
10271 676 2008 13 PDF Available
Title
The effect of enzymatically degradable poly(ethylene glycol) hydrogels on smooth muscle cell phenotype
Abstract

The formation of scar tissue due to dedifferentiation of smooth muscle cells (SMCs) is one of the major issues faced when engineering bladder tissue. Furthermore, cell sources for regenerating the SMC layer are also limiting. Here we explore if human mesenchymal stem cells (MCSs), cultured in enzymatically degradable poly(ethylene glycol) (PEG) hydrogel scaffolds can be differentiated into SMC-like cells. We explored the degree to which a less synthetic SMC phenotype can be achieved when primary human SMCs are cultured within these scaffolds, It was observed that when both MSCs and SMCs are cultured in the PEG hydrogel scaffolds, but not on traditional tissue culture plastic, they up-regulate markers associated with the less synthetic SMC phenotype, decreased expression of α5 integrin and THY-1, and increased expression of α-smooth muscle actin (αSMA) and myosin. Furthermore, we show that MSCs and SMCs cultured in the PEG hydrogels are able to proliferate and express matrix metalloproteinases for up to 21 d in culture, the duration of the study. This study addresses the importance of the cellular microenvironment on cell fate, and proposes synthetic instructive biomaterials as a means to direct cell differentiation and circumvent scar tissue formation during bladder reconstruction.

Keywords
Hydrogel; PEG; ECM; Bladder; Mesenchymal stem cell; Smooth muscle cell
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The effect of enzymatically degradable poly(ethylene glycol) hydrogels on smooth muscle cell phenotype
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 3, January 2008, Pages 314–326
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us