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Positioning and guidance of neurons on gold surfaces by directed assembly of proteins using Atomic Force Microscopy

Paper ID Volume ID Publish Year Pages File Format Full-Text
10296 678 2009 8 PDF Available
Title
Positioning and guidance of neurons on gold surfaces by directed assembly of proteins using Atomic Force Microscopy
Abstract

We demonstrate that Atomic Force Microscopy nanolithography can be used to control effectively the adhesion, growth and interconnectivity of cortical neurons on Au surfaces. We demonstrate immobilization of neurons at well-defined locations on Au surfaces using two different types of patterned proteins: 1) poly-d-lysine (PDL), a positively charged polypeptide used extensively in tissue culture and 2) laminin, a component of the extracellular matrix. Our results show that both PDL and laminin patterns can be used to confine neuronal cells and to control their growth and interconnectivity on Au surfaces, a significant step towards the engineering of artificial neuronal assemblies with well-controlled neuron position and connections.

Keywords
AFM; Microfabrication; Protein patterning; Cortical neurons; Self-assembled monolayers; Neural network
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Positioning and guidance of neurons on gold surfaces by directed assembly of proteins using Atomic Force Microscopy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 20, July 2009, Pages 3397–3404
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us