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Temporal and spatially controllable cell encapsulation using a water-soluble phospholipid polymer with phenylboronic acid moiety

Paper ID Volume ID Publish Year Pages File Format Full-Text
10312 679 2007 8 PDF Available
Title
Temporal and spatially controllable cell encapsulation using a water-soluble phospholipid polymer with phenylboronic acid moiety
Abstract

Temporal and spatially controllable cell encapsulation based on a water-soluble phospholipid polymer is reported in this study. Phospholipid polymers, i.e., poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-vinylphenylboronic acid) (PMBV), were synthesized. A series of hydrogels was prepared between the water-soluble PMBV and other water-soluble polymers having multi-valent alcoholic groups, such as poly(vinyl alcohol) (PVA). The PMBV/PVA hydrogels were formed not only in water, but also in a cell culture medium, and dissociated by the excess addition of low molecular weight di-valent hydroxyl compounds, such as d-glucose. The PMBV/PVA hydrogel was applied as a cell-container which has three-dimensional matrices for the reversible encapsulation of living cells without any response in it. Uniform cell seeding can be achieved using the hydrogels due to the homogenous gel formation of PMBV and PVA in the cell culture medium. Fibroblast cells were encapsulated in the PMBV/PVA hydrogel and maintained for 1 week. After dissociation of the PMBV/PVA hydrogel, the cells were seeded on conventional tissue culture polystyrene. The cells adhered and proliferated as usual on the plate. That is, the PMBV/PVA hydrogel will be useful as a cell-container, which can maintain the cells without any significant adverse effect on the entrapped cells.

Keywords
Phospholipid polymer; Cell engineering; Polymer complex; Reversible hydrogel; Cytocompatibility; Encapsulation
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Temporal and spatially controllable cell encapsulation using a water-soluble phospholipid polymer with phenylboronic acid moiety
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 10, April 2007, Pages 1770–1777
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us