fulltext.study @t Gmail

In vitro effect of oligo-hydroxyalkanoates on the growth of mouse fibroblast cell line L929

Paper ID Volume ID Publish Year Pages File Format Full-Text
10331 680 2007 8 PDF Available
Title
In vitro effect of oligo-hydroxyalkanoates on the growth of mouse fibroblast cell line L929
Abstract

The cellular responses to polyhydroxyalkanoates (PHA) degradation products oligo-hydroxyalkanoates (OHAs) are very important factors that control the biocompatibility of these polymers when they are used in tissue-engineering applications. In this study, oligo(3-hydroxybutyrate) (OHB, Mn 2000), oligo(3-hydroxybutyrate-co-4-hydroxybutyrate) (O3HB4HB, Mn 2100, 6 mol% 4HB), oligo(3-hydroxybutyrate-co-3-hydroxyhexanoate) (OHBHHx, Mn 2800, 12 mol% 3HHx) and medium-chain-length oligo(3-hydroxyalkanoates) (OmclHAs, Mn 2300, 2 mol% 3-hydroxyhexanoate (3HHx), 25 mol% 3-hydroxyoctanoate, 71 mol% 3-hydroxydecanoate and 3 mol% 3-hydroxydodecanoate) were prepared as insoluble particles in aqueous solution. Liposomes were employed to encapsulate OHAs and facilitate their transfer into the cytosol. The methylthiazol tetrazolium (MTT) assay, a cell apoptosis study and the flow cytometry were used to evaluate cell viability of mouse fibroblast L929. OHAs in concentration lower than 20 mg/l did not significantly affect cell viability, while OHAs over 40 mg/l reduced cell viability with more cell apoptosis, more cell death, delayed cell cycle and reduced cell proliferation. The cytotoxicity of OHAs decreased with increasing OHAs side chain length. It is proposed that medium-chain-length OHAs containing PHA, such as poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) and medium-chain-length PHA Poly(3-hydroxyalkanoates) (mcl PHA), are preferred for development and application as PHA-based tissue-engineering biomaterials.

Keywords
PHB; Polyhydroxyalkanoates; Oligo-hydroxyalkanoates; Tissue engineering; Biomaterials; Liposome
First Page Preview
In vitro effect of oligo-hydroxyalkanoates on the growth of mouse fibroblast cell line L929
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 27, September 2007, Pages 3896–3903
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us