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The use of biotin–avidin binding to facilitate biomodification of thermoresponsive culture surfaces

Paper ID Volume ID Publish Year Pages File Format Full-Text
10352 681 2007 6 PDF Available
Title
The use of biotin–avidin binding to facilitate biomodification of thermoresponsive culture surfaces
Abstract

Here, we report biomodification of temperature-responsive culture surfaces with biotinylated biomolecules utilizing streptavidin and biotinylation of the surfaces. Poly(N-isopropylacrylamide-co-2-carboxyisopropylacrylamide) was covalently grafted onto tissue culture polystyrene (TCPS) dishes. Biotinylated Arg-Gly-Asp-Ser (RGDS) peptides with different spacer lengths (biotin-conjugated GnRGDS (n=1, 6, 12, 16)) were examined. Human umbilical vein endothelial cells (HUVECs) adhered and were well spread on G12RGDS-immobilized surfaces in the absence of serum at 37 °C, while much less cell adhesion was observed with the other peptides. Adhered HUVECs were detached on reducing temperature to 20 °C, or on adding free RGDS peptide. Interestingly, cell detachment was accelerated by applying both these techniques. Consequently, by optimizing the spacer length, biomolecules can be functionally immobilized onto thermoresponsive surfaces via the affinity binding between avidin and biotin.

Keywords
Temperature-responsive; Cell culture; Biofunctionalization; RGDS
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The use of biotin–avidin binding to facilitate biomodification of thermoresponsive culture surfaces
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 36, December 2007, Pages 5471–5476
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us