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Long-term neurite orientation on astrocyte monolayers aligned by microtopography

Paper ID Volume ID Publish Year Pages File Format Full-Text
10355 681 2007 11 PDF Available
Title
Long-term neurite orientation on astrocyte monolayers aligned by microtopography
Abstract

After spinal cord injury neuronal connections are not easily re-established. Success has been hampered by the lack of orientation of neurites inside scar tissue and a lack of neurites crossing out of the site of injury. Oriented scaffolds in biodegradable polymers could be an excellent way to support both the orientation of neurites within the injury site as well as aiding their crossing out of the lesion. To establish the validity of using grooved micro-topography in polycaprolactone in combination with glia we have studied the long-term (3 weeks) orientation of neuronal cells on monolayers of astrocytes on the top of grooved topographies of various dimensions. We find that neurites are significantly aligned by groove/ridge type topographies which are “buried” under a monolayer of astrocytes for up to 3 weeks. This alignment is significantly lower than that of neurites growing directly on the topography, but these neurons do not survive on the poly-l-lysine coated polymer for more than a week. The alignment of neurites on the astrocyte layer to the underlying topography decreases over time, and with groove width. Topographies with 12.5 or 25 μm lateral dimension appear optimal for the long-term alignment and can support myelination. We have shown for the first time that micro-topography can act through an overlaid astrocyte layer and results in aligned neurites in long-term culture and that these can be myelinated by endogenous oligodendrocytes.

Keywords
Astrocytes; Nerve guide; Myelination; Topography
First Page Preview
Long-term neurite orientation on astrocyte monolayers aligned by microtopography
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 36, December 2007, Pages 5498–5508
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering