fulltext.study @t Gmail

Biospecific anchoring and spatially confined germination of bacterial spores in non-biofouling microwells

Paper ID Volume ID Publish Year Pages File Format Full-Text
10365 681 2007 7 PDF Available
Title
Biospecific anchoring and spatially confined germination of bacterial spores in non-biofouling microwells
Abstract

In this paper, we report a simple method for spatially confining Bacillus subtilis (BS) spores into semi-three dimensional, non-biofouling microwells by using biospecific (such as biotin–streptavidin) interactions. Non-biofouling poly(ethylene glycol) (PEG)-based microwells were fabricated by employing a process of capillary molding on a glass slide. The biospecific interactions between biotinylated BS spores and streptavidin led to the selective deposition of BS spores onto the bottom of the microwells of which presented streptavidin. The viability of the patterned spores was confirmed by the induction of germination. Bacterial spores were found to maintain extreme robustness until they were exposed to favorable conditions. This work suggests that the use of bacterial spore-based sensors would increase the shelf-life (such as long-term storage and stability) of cell-based sensors.

Keywords
Spore; Vegetative cells; Microwells; Biospecific interactions; Patterning
First Page Preview
Biospecific anchoring and spatially confined germination of bacterial spores in non-biofouling microwells
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 36, December 2007, Pages 5594–5600
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering