fulltext.study @t Gmail

The effect of water-soluble chitosan on macrophage activation and the attenuation of mite allergen-induced airway inflammation

Paper ID Volume ID Publish Year Pages File Format Full-Text
10369 682 2008 10 PDF Available
Title
The effect of water-soluble chitosan on macrophage activation and the attenuation of mite allergen-induced airway inflammation
Abstract

Chitin and chitosan have versatile anti-tumor, anti-fungal, and antimicrobial biological properties. Oral intakes and intranasal administration of chitin attenuated allergen-induced airway inflammation in sensitized mice, which may be due to its Th1 adjuvant properties. However, their mechanism of action is not entirely clear. In this report, we demonstrate that water-soluble chitosan (WSC) has specific immunomodulatory effects on dust mite allergen Dermatophagoides farinae (Der f)-stimulated, monocyte-derived macrophages (MDM). These effects include polarizing the cytokine balance towards Th1 cytokines, decreasing the production of the inflammatory cytokines IL-6 and TNF-α, down-regulating CD44 and TLR4 receptor expression, and inhibiting T cell proliferation. Scanning electron microscope (SEM) examination found that WSC reduced the rate of pseudopodia formation in Der f-stimulated MDM from allergic asthma patients. The effect of WSC on allergen-stimulated MDM may be mediated via inhibition of PKCζ phosphorylation and NF-κB pathway activation. In a murine model of asthma, we found that intranasal application of WSC attenuates Der f-induced lung inflammation by reducing infiltration of inflammatory cells, epithelial damage, and goblet cell hyperplasia and markedly decreasing production of Arg I, iNOs, and thymic stromal lymphopoietin (TSLP) in the bronchial epithelium. Therefore, we believe that WSC may provide a new therapeutic modality for allergic asthma.

Keywords
Macrophage; Chitosan; AllergyArg I, arginase I; Der f, Dermatophagoides farinae; Der p, Dermatophagoides pteronyssinus; iNOs, inducible nitric oxide synthase; HDM, house dust mite; MDM, monocyte-derived macrophages; PBMCs, peripheral blood mononuclear cel
First Page Preview
The effect of water-soluble chitosan on macrophage activation and the attenuation of mite allergen-induced airway inflammation
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 14, May 2008, Pages 2173–2182
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us