fulltext.study @t Gmail

Inhibition of in vitro chondrogenesis in RGD-modified three-dimensional alginate gels

Paper ID Volume ID Publish Year Pages File Format Full-Text
10425 684 2007 13 PDF Available
Title
Inhibition of in vitro chondrogenesis in RGD-modified three-dimensional alginate gels
Abstract

The goal of this study was to investigate the effects of adhesion to the arginine-glycine-aspartic acid (RGD) sequence on the chondrogenesis of bone marrow stromal cells (BMSCs). Synthetic RGE- and RGD-containing peptides were conjugated to sodium alginate, and bovine BMSCs were seeded onto 2D alginate surfaces or encapsulated in 3D gels. BMSCs spread specifically on RGD-modified surfaces, and spreading was inhibited by a soluble RGD peptide and by anti-β1 and anti-αvβ3 integrin blocking antibodies. After 7 days in 3D gel culture, the chondrogenic supplements (TGF-β1 and dexamethasone) significantly stimulated chondrocytic gene expression (collagen II, aggrecan, and Sox-9) and matrix accumulation (collagen II and sGAG) in RGE-modified gels, but this response was inhibited in the RGD-modified gels. Inhibition of sGAG synthesis increased with increasing RGD density, and synthesis was partially rescued by adding a soluble RGD peptide. Addition of an anti-αvβ3 integrin blocking antibody had no effect on chondrogenesis, while an anti-α5 antibody reduced sGAG accumulation. Overall, this study demonstrates that interaction with the RGD motif significantly inhibits the initial chondrogenesis of BMSCs within 3D alginate gels. These results provide new insights into the role of cell–matrix interactions in regulating chondrogenesis and highlight the importance of choosing appropriate biomaterials for tissue engineering therapies.

Keywords
Alginate; Cartilage tissue engineering; Mesenchymal stem cell; Integrin; RGD peptide; TGF
First Page Preview
Inhibition of in vitro chondrogenesis in RGD-modified three-dimensional alginate gels
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 6, February 2007, Pages 1071–1083
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering