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Temporal differences in Erk1/2 activity distinguish among combinations of extracellular matrix components

Paper ID Volume ID Publish Year Pages File Format Full-Text
1044 72 2011 8 PDF Available
Title
Temporal differences in Erk1/2 activity distinguish among combinations of extracellular matrix components
Abstract

Rational design of biomaterials requires understanding how cells interrogate their microenvironment. In this study, human umbilical vein endothelial cells are cultured on combinations of extracellular matrix (ECM) components (collagen I, collagen IV, vitronectin, fibronectin, laminin, heparan sulfate proteoglycan, chondroitin sulfate proteoglycan), and the phosphorylation of four intracellular signaling kinases (Erk1/2, JNK, Akt1, and NFκB) is quantified. These combinations of ECM components elicit different temporal patterns of Erk1/2 phosphorylation. Collagen I-containing substrates cause Erk1/2 phosphorylation to reach maximal levels at 30 min and remain near maximal levels until 90 min. Collagen IV/laminin substrates elicit maximal phosphorylation at 30–45 min, and then phosphorylation decreases substantially at 60–90 min. All other combinations studied (collagen IV and vitronectin-based combinations) cause an increase in phosphorylation at 30–45 min, but not to maximal levels; maximal phosphorylation is reached by 60–90 min. These temporal patterns of phosphorylation may explain how a limited number of intracellular signaling pathways can distinguish among thousands of possible combinations of microenvironmental cues by adding to the information contained in each cell signaling pathway.

Keywords
Endothelial cells; Intracellular signaling; Biomaterial; Basement membrane; Systems biology
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Temporal differences in Erk1/2 activity distinguish among combinations of extracellular matrix components
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 11, November 2011, Pages 3973–3980
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us