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The influence of polymeric properties on chitosan/siRNA nanoparticle formulation and gene silencing

Paper ID Volume ID Publish Year Pages File Format Full-Text
10446 684 2007 9 PDF Available
Title
The influence of polymeric properties on chitosan/siRNA nanoparticle formulation and gene silencing
Abstract

We have previously introduced the use of the biomaterial chitosan to form chitosan/siRNA nanoparticles for gene silencing protocols. This present study shows that the physicochemical properties (size, zeta potential, morphology and complex stability) and in vitro gene silencing of chitosan/siRNA nanoparticles are strongly dependent on chitosan molecular weight (Mw) and degree of deacetylation (DD). High Mw and DD chitosan resulted in the formation of discrete stable nanoparticles ∼200 nm in size. Chitosan/siRNA formulations (N:P 50) prepared with low Mw (∼10 kDa) showed almost no knockdown of endogenous enhanced green fluorescent protein (EGFP) in H1299 human lung carcinoma cells, whereas those prepared from higher Mw (64.8–170 kDa) and DD (∼80%) showed greater gene silencing ranging between 45% and 65%. The highest gene silencing efficiency (80%) was achieved using chitosan/siRNA nanoparticles at N:P 150 using higher Mw (114 and 170 kDa) and DD (84%) that correlated with formation of stable nanoparticles of ∼200 nm. In conclusion, this work confirms the application of chitosan as a non-viral carrier for siRNA and the importance of polymeric properties for the optimisation of gene silencing using chitosan/siRNA nanoparticles.

Keywords
RNA interference; Chitosan; siRNA; Nanoparticle; Delivery; Gene silencing
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The influence of polymeric properties on chitosan/siRNA nanoparticle formulation and gene silencing
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 6, February 2007, Pages 1280–1288
Authors
, , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us