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Kinetic analysis of nanoparticulate polyelectrolyte complex interactions with endothelial cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
10459 685 2007 13 PDF Available
Title
Kinetic analysis of nanoparticulate polyelectrolyte complex interactions with endothelial cells
Abstract

A non-toxic, nanoparticulate polyelectrolyte complex (PEC) drug delivery system was formulated to maintain suitable physicochemical properties at physiological pH. Toxicity, binding, and internalization were evaluated in relevant microvascular endothelial cells. PEC were non-toxic, as indicated by cell proliferation studies and propidium iodide staining. Inhibitor studies revealed that PEC were bound, in part, via heparan sulfate proteoglycans and internalized through macropinocytosis. A novel, flow cytometric, Scatchard protocol was established and showed that PEC, in the absence of surface modification, bind cells non-specifically with positive cooperativity, as seen by graphical transformations.

Keywords
Polyelectrolyte complexes; Flow cytometry; Scatchard plots; Nanoparticles; Drug delivery
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Kinetic analysis of nanoparticulate polyelectrolyte complex interactions with endothelial cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 26, September 2007, Pages 3843–3855
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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Any Questions? feel free to contact us