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Silk fibroin microtubes for blood vessel engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
10470 686 2007 9 PDF Available
Title
Silk fibroin microtubes for blood vessel engineering
Abstract

Currently available synthetic grafts demonstrate moderate success at the macrovascular level, but fail at the microvascular scale (<6 mm inner diameter). We report on the development of silk fibroin microtubes for blood vessel repair with several advantages over existing scaffold materials/designs. These microtubes were prepared by dipping straight lengths of stainless steel wire into aqueous silk fibroin, where the addition of poly(ethylene oxide) (PEO) enabled control of microtube porosity. The microtube properties were characterized in terms of pore size, burst strength, protein permeability, enzymatic degradation, and cell migration. Low porosity microtubes demonstrated superior mechanical properties in terms of higher burst pressures, but displayed poor protein permeability; whereas higher porosity tubes had lower burst strengths but increased permeability and enhanced protein transport. The microtubes also exhibited cellular barrier functions as low porosity tubes prevented outward migration of GFP-transduced HUVECs, while the high porosity microtubes allowed a few cells per tube to migrate outward during perfusion. When combined with the biocompatible and suturability features of silk fibroin, these results suggest that silk microtubes, either implanted directly or preseeded with cells, are an attractive biomaterial for microvascular grafts.

Keywords
Silk fibroin; Vascular grafts; Arterial tissue engineering; Porosity; Endothelial cells; Bioreactor
First Page Preview
Silk fibroin microtubes for blood vessel engineering
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 35, December 2007, Pages 5271–5279
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering