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Interactions of antigen-loaded polylactide particles with macrophages and their correlation with the immune response

Paper ID Volume ID Publish Year Pages File Format Full-Text
10477 686 2007 14 PDF Available
Title
Interactions of antigen-loaded polylactide particles with macrophages and their correlation with the immune response
Abstract

Size of the polymeric particulate antigen delivery system and its interactions with antigen-presenting cells (APCs) influence the immune response both qualitatively and quantitatively. In this paper, we report that antigen-loaded polymeric microparticles elicit antibody titers without being phagocytosed by macrophages; and size of the antigen-loaded particles modulates immune response from single-point immunization. Antibody titers varied significantly from single-point immunization with different sized polylactide (PLA) particles entrapping hepatitis B surface antigen. Nanoparticles (200–600 nm) were efficiently taken up by macrophages and elicited lower antibody titers in comparison to microparticles (2–8 μm). PLA microparticles that elicited highest and long-lasting antibody titers from single-point immunization were not taken up by the macrophages and found attached to the surface of the macrophages. Immunization with nanoparticles (200–600 nm) was associated with higher levels of IFN-γ production, upregulation of MHC class I molecules along with antibody isotypes favoring Th1-type immune response. Immunization with microparticles (2–8 μm size) promoted IL-4 secretion, upregulated MHC class II molecules and favored Th2-type immune response. Western blot analysis showed that release of HBsAg from surface-attached microparticles into macrophages increased with time, but was more or less constant in case of nanoparticles. Our results suggest that continuous release of high concentration of antigen from cell surface-attached PLA microparticles into APCs results in improved antibody response from single-point immunization. It also offers an exciting possibility of designing size-based polymer particle delivery system to modulate immune response.

Keywords
Polylactide; Microparticles; Nanoparticles; Macrophage; Cellular interaction; Immune response
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Interactions of antigen-loaded polylactide particles with macrophages and their correlation with the immune response
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 35, December 2007, Pages 5344–5357
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us