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Electrospun nanofiber scaffolds for rapid and rich capture of bone marrow-derived hematopoietic stem cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
10493 687 2008 8 PDF Available
Title
Electrospun nanofiber scaffolds for rapid and rich capture of bone marrow-derived hematopoietic stem cells
Abstract

Interactions between bone marrow-derived hematopoietic stem cells (BM-HSCs) and their local microenvironment are an integral part of signaling control of BM-HSCs migration, proliferation and differentiation. We hypothesized that both substrate topographical and biochemical cues promote BM-HSCs adhesive behaviors, which are crucial for BM-HSCs' homing, self-renewal and lineage commitment within their microenvironment. We employed electrospinning technique to fabricate nanofiber scaffolds (NFS) with poly(dl-lactide-co-glycolide) blended with collagen I. NFS was further coated with E-selectin, a critical adhesive biomolecule. Capture efficiency study showed that blended NFS, after coated with E-selectin, significantly increased cell capture percentage from 23.40% to 67.41% within 30 min and from 29.44% to 70.19% within 60 min of incubation at room temperature. This study highlights the potential of using a biomimetic scaffold to design a site-specific niche-like unit for facilitating proliferation or differentiation functions of BM-HSCs.

Keywords
Electrospinning; Nanofiber; Hematopoietic stem cells; Cell adhesion
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Electrospun nanofiber scaffolds for rapid and rich capture of bone marrow-derived hematopoietic stem cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 13, May 2008, Pages 2096–2103
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us