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Imprinting effect of protein-imprinted polymers composed of chitosan and polyacrylamide: A re-examination

Paper ID Volume ID Publish Year Pages File Format Full-Text
10497 687 2008 5 PDF Available
Title
Imprinting effect of protein-imprinted polymers composed of chitosan and polyacrylamide: A re-examination
Abstract

In this paper the imprinting effects recently reported with protein-imprinted polymers based on chitosan and polyacrylamide are re-assessed. Molecularly imprinted polymers (MIPs) from which the embedded template molecules were removed by washing with a solution of sodium dodecyl sulfate and acetic acid were prepared. In batch template rebinding experiments, the MIPs displayed quite high template binding capacity as reported previously. However, the non-imprinted polymers (NIPs), after washing with the same solution, also showed large binding capacity nearly equal to that of the MIPs. X-ray diffraction and scanning electronic microscope investigations confirmed remarkable property changes of the NIPs after the washing process. These findings indicate that the non-specific adsorption resulting from the template removing process rather than the imprinted sites generated on the MIPs themselves may account for the high template rebinding capacity of the reported protein-imprinted polymers.

Keywords
Molecular imprinting; Protein; Protein adsorption; Chitosan; Hydrogel; Polyacrylamide
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Imprinting effect of protein-imprinted polymers composed of chitosan and polyacrylamide: A re-examination
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 13, May 2008, Pages 2138–2142
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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