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Islet encapsulation with living cells for improvement of biocompatibility

Paper ID Volume ID Publish Year Pages File Format Full-Text
10511 688 2009 6 PDF Available
Title
Islet encapsulation with living cells for improvement of biocompatibility
Abstract

Bioartificial pancreas, microencapsulation of islets of Langerhans (islets) within devices has been studied as a safe and simple technique for islet transplantation without the need for immuno-suppressive therapy. Various types of bioartificial pancreas have been proposed and developed such as microcapsule, macrocapsule and diffusion chamber types. However, these materials comprising a bioartificial pancreas are not completely inert and may induce foreign body and inflammatory reactions. The residual materials would be a problem in human body. Here we propose an alternative method for microencapsulation of islets with a layer of living cells. We immobilized HEK293 cells (human endoderm kidney cell line) to the islet surface using amphiphilic poly(ethylene glycol)-conjugated phospholid derivative and biotin/streptavidin reaction and encapsulated islets with a cell layer by culture. No necrosis of islet cells at the center was seen after microencapsulation with a layer of living cells. Insulin secretion ability by glucose stimulation was well maintained on these cell-encapsulated islets.

Keywords
Bioartificial pancreas; Islet; Cell-encapsulation; Microencapsulation; Poly(ethylene glycol)–lipid (PEG–lipid)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 12, April 2009, Pages 2270–2275
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us