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Bienzyme functionalized three-layer composite magnetic nanoparticles for electrochemical immunosensors

Paper ID Volume ID Publish Year Pages File Format Full-Text
10513 688 2009 7 PDF Available
Title
Bienzyme functionalized three-layer composite magnetic nanoparticles for electrochemical immunosensors
Abstract

The preparation, characterization and application of a three-layer magnetic nanoparticle composed of an Fe3O4 magnetic core, a Prussian Blue (PB) interlayer and a gold shell (it can be abbreviated as Au–PB–Fe3O4) for an ultrasensitive and reproducible electrochemical immunosensing fabrication were described for the first time in this work. With the employment of the Au–PB–Fe3O4 nanoparticle, a new signal amplification strategy was developed based on bienzyme (horseradish peroxidase and glucose oxidase) functionalized Au–PB–Fe3O4 nanoparticles for an electrochemical immunosensing fabrication by using Carcinoembryonic antigen (CEA) and α-fetoprotein (AFP) as model systems, respectively. The experiment results show that the multilabeled Au–PB–Fe3O4 nanoparticles exhibit satisfying redox electrochemical activity and high enzyme catalysis activity, which predetermine their utility in high sensitivity antibody detection schemes. Furthermore, this immunosensor could be regenerated by simply using an external magnetic field which ensured a reproducible immunosensor with high sensitivity.

Keywords
Magnetic nanocomposite; Biosensor; Enzyme; Electrochemistry
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 12, April 2009, Pages 2284–2290
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us