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Volumetric interpretation of protein adsorption: Partition coefficients, interphase volumes, and free energies of adsorption to hydrophobic surfaces

Paper ID Volume ID Publish Year Pages File Format Full-Text
10532 689 2006 14 PDF Available
Title
Volumetric interpretation of protein adsorption: Partition coefficients, interphase volumes, and free energies of adsorption to hydrophobic surfaces
Abstract

The solution-depletion method of measuring protein adsorption is implemented using SDS gel electrophoresis as a separation and quantification tool. Experimental method is demonstrated using lysozyme (15 kDa), α-amylase (51 kDa), human serum albumin (66 kDa), prothrombin (72 kDa), immunoglobulin G (160 kDa), and fibrinogen (341 kDa) adsorption from aqueous-buffer solution to hydrophobic octyl-sepharose and silanized-glass particles. Interpretive mass-balance equations are derived from a model premised on the idea that protein reversibly partitions from bulk solution into a three-dimensional (3D) interphase volume separating the physical-adsorbent surface from bulk solution. Theory both anticipated and accommodated adsorption of all proteins to the two test surfaces, suggesting that the underlying model is descriptive of the essential physical chemistry of protein adsorption. Application of mass balance equations to experimental data quantify partition coefficients P, interphase volumes VI, and the number of hypothetical layers M occupied by protein adsorbed within VI. Partition coefficients quantify protein-adsorption avidity through the equilibrium ratio of interphase and bulk-solution-phase w/v (mg/mL) concentrations WI and WB, respectively, such that P≡WI/WBP≡WI/WB. Proteins are found to be weak biosurfactants with 45

Keywords
Protein adsorption; Hydrophobic solid–liquid interface; Globular blood proteins; Octyl sepharose
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Volumetric interpretation of protein adsorption: Partition coefficients, interphase volumes, and free energies of adsorption to hydrophobic surfaces
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 34, December 2006, Pages 5780–5793
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
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