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Real-time analysis of cell–surface adhesive interactions using thickness shear mode resonator

Paper ID Volume ID Publish Year Pages File Format Full-Text
10535 689 2006 8 PDF Available
Title
Real-time analysis of cell–surface adhesive interactions using thickness shear mode resonator
Abstract

The cell adhesion process and the molecular interactions that determine its kinetics were investigated using a thickness shear mode (TSM) sensor. The goal of this study was to correlate sensor readings with the progression of cell adhesion. In particular, the specific effects of receptor-mediated adhesion, the glycocalyx, and surface charge on initial cell–surface attachment and steady-state adhesion of endothelial cells were investigated. We found a strong correlation between resistance changes (ΔR) and the development of cell adhesion strength by comparing the sensor readings with independently assessed cell adhesion. The result showed that integrin binding determines the kinetics of initial cell attachment while heparan sulfate proteoglycan (HSPG) modulates steady-state adhesion strength. Coating the sensor surface with the positively charged poly-d-lysine (PDL) enhanced the initial interaction with substratum. These data confirm our current understanding of the contribution of these three phenomena to the adhesion process. The real-time monitoring capability of this technique with high temporal resolution provides more detailed information on the kinetics of the different stages of the adhesion process. This technique has the potential to facilitate the evaluation of biomaterials and surface treatments used for implants and tissue-engineering scaffolds for their bioactive effects on the cell adhesion process.

Keywords
Cell adhesion; Biosensor; Endothelial cell; Surface treatment; Integrin; RGD peptide
First Page Preview
Real-time analysis of cell–surface adhesive interactions using thickness shear mode resonator
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 34, December 2006, Pages 5813–5820
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering