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Graded delamination behavior of human stratum corneum

Paper ID Volume ID Publish Year Pages File Format Full-Text
10541 689 2006 10 PDF Available
Title
Graded delamination behavior of human stratum corneum
Abstract

An in vitro adhesion test method has been adapted to quantify the through-thickness intercellular delamination energy of isolated human stratum corneum (SC). Both untreated and delipidized tissues were tested. Measured delamination energies were found to increase from ∼3 J/m2 near the surface to ∼15 J/m2 for the inner layers of the tissue. For delipidized SC, the location of the initial debond was located closer to the center of the tissue. Delamination energy values were elevated compared to untreated specimens, increasing from ∼7 J/m2 near the surface to ∼18 J/m2 for the inner layers of the SC. Further tests were run to measure delamination energies of SC as a function of hydration (15–100% relative humidity (RH)) at ∼25 °C and as a function of temperature (10–90 °C) at several hydrations (15, 45, 100% RH). Delamination energies were observed to decrease with increasing hydration and increasing temperature with the most significant changes occurring for 100% RH conditioned SC. Additional SC was treated with pH-buffered solutions (pH 4.2, 6.7, 9.9) and selected surfactant solutions (1%, 10% wt/wt sodium dodecyl sulfate (SDS)) for comparison to untreated controls. While statistically significant differences were observed, the SC was found to be resistant to large changes in delamination energy with pH and 1% wt/wt SDS treatments with values in the range 4.2–5.1 J/m2 compared to control values of 4.4 J/m2. More substantially elevated values were observed for SC treated with a 10% wt/wt SDS solution (6.6 J/m2) and a chloroform–methanol extraction (11.2 J/m2).

Keywords
Mechanical properties; Fracture toughness; Epithelial cell; Stratum corneum; Tissue treatmentSC, stratum corneum; RH, relative humidity; FH, fully hydrated; DCB, double-cantilever beam; CMT, chloroform–methanol treated; SDS, sodium dodecyl sulfate; FTIR,
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 34, December 2006, Pages 5861–5870
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us