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Neural probe design for reduced tissue encapsulation in CNS

Paper ID Volume ID Publish Year Pages File Format Full-Text
10545 690 2007 14 PDF Available
Title
Neural probe design for reduced tissue encapsulation in CNS
Abstract

This study investigated relationships between a microscale neural probe's size and shape and its chronic reactive tissue response. Parylene-based probes were microfabricated with a thick shank (48 μm by 68 μm) and an integrated thin lateral platform (5 μm by 100 μm, either solid or one of three lattice sizes). Devices were implanted in rat cerebral cortex for 4 weeks before immunostaining for neurons, astrocytes, microglia, fibronectin, laminin, and neurofilament. While nonneuronal density (NND) generally increased and neuronal density decreased within 75 μm of a probe interface compared to unimplanted control regions, there were significant differential tissue responses within 25 μm of the platform's lateral edge compared to the shank. The NND in this region of the lateral edge was less than one-third of the corresponding region of the shank (129% and 425% increase, respectively). Moreover, neuronal density around the platform lateral edge was about one-third higher than at the shank (0.70 and 0.52 relative to control, respectively). Also, microglia reactivity and extracellular protein deposition was reduced at the lateral edge. There were no significant differences among platform designs. These results suggest that neural probe geometry is an important parameter for reducing chronic tissue encapsulation.

Keywords
Biosensor; Cell encapsulation; Foreign body response; Neural prosthesis
First Page Preview
Neural probe design for reduced tissue encapsulation in CNS
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 25, September 2007, Pages 3594–3607
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering