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The effect of 3-hydroxybutyrate and its derivatives on the growth of glial cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
10546 690 2007 9 PDF Available
Title
The effect of 3-hydroxybutyrate and its derivatives on the growth of glial cells
Abstract

Sodium salts of d-3-hydroxybutyrate (D-3-HB), dl-3-hydroxybutyrate (DL-3-HB) and methyl (d)-3-hydroxybutyrate (M-3-HB), are derivatives of 3-hydroxybutyric acid (3-HB), a ketone body that is produced in vivo in animals including human. D-3-HB is the most common degradation product of microbial polyhydroxyalkanoates (PHA) that have been investigated for tissue engineering application. This study evaluated the in vitro effect of PHA degradation product 3-HB and its derivatives (collectively called 3-HB derivatives) on cell apoptosis and cytosolic Ca2+ concentration of mouse glial cells. Results showed that the percentage of cells undergoing apoptosis decreased significantly in the presence of 3-HB and its derivatives as evidenced by flow cytometry. The in vitro study on cytosolic Ca2+ concentration demonstrated that 3-HB derivatives dramatically elevated the cytosolic Ca2+ concentration. Both the extracellular and intracellular Ca2+ contributed as the sources of such Ca2+ concentration elevation. The effect of 3-HB derivatives on cytosolic Ca2+ concentration could be reduced by nitredipine, an l-type voltage-dependent calcium channel antagonist. In comparison, M-3-HB worked more efficiently than D-3-HB and DL-3-HB did as M-3-HB is more efficient in permeation into the cells. All results indicated that 3-HB derivatives had an inhibitory effect on cell apoptosis which is mediated by signaling pathways related to the elevation of cytosolic Ca2+ concentration. This positive effect helps explain the biocompatibility observed for PHA, it also points to the possibility of 3-HB derivatives regardless of chirality to become an effective neural protective agent.

Keywords
3-HB; PHB; Apoptosis; Cytosolic Ca2+ concentration; Glial cell; Polyhydroxyalkanoates
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The effect of 3-hydroxybutyrate and its derivatives on the growth of glial cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 25, September 2007, Pages 3608–3616
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us