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Patterned biofunctional designs of thermoresponsive surfaces for spatiotemporally controlled cell adhesion, growth, and thermally induced detachment

Paper ID Volume ID Publish Year Pages File Format Full-Text
10549 690 2007 12 PDF Available
Title
Patterned biofunctional designs of thermoresponsive surfaces for spatiotemporally controlled cell adhesion, growth, and thermally induced detachment
Abstract

In the present study, we report advanced patterned biofunctionalization of thermoresponsive surfaces for achievement of spatiotemporally controlled cell adhesion, growth, and thermally induced detachment. These patterned biofunctional thermoresponsive surfaces were prepared using dual surface modification techniques: electron beam-induced surface patterning of carboxyl-functional thermoresponsive polymers with appropriate metal masks and following site-selective biofunctionalization with biomolecules, the cell adhesive peptide (RGDS) and/or the cell growth factor (insulin; INS). Patterned co-immobilization of RGDS–INS onto thermoresponsive surfaces dominated site-selective cell adhesion and growth along with patterned biofunctional domains in the serum-free culture. Spatiotemporal detachment of sparsely adherent and confluent cells from these patterned biofunctional thermoresponsive surfaces were both achieved only by reducing temperature. Furthermore, RGDS–INS-patterned thermoresponsive surfaces also successfully demonstrated the selective fabrication and recovery of either contiguous monolayer or mesh-like designed monolayer tissue constructs on the identical surfaces. Thus, patterned biofunctional designs would be utilized for the creation and harvest of biomimetic-designed vascular networks having sufficient biofunctional activities in facilitated cell sheet engineering and regenerative medicine.

Keywords
Patterned biofunctional thermoresponsive surfaces; RGDS; Insulin (INS); Spatiotemporal control; Designed monolayer tissues
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Patterned biofunctional designs of thermoresponsive surfaces for spatiotemporally controlled cell adhesion, growth, and thermally induced detachment
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 25, September 2007, Pages 3632–3643
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us