fulltext.study @t Gmail

Upregulation of bone cell differentiation through immobilization within a synthetic extracellular matrix

Paper ID Volume ID Publish Year Pages File Format Full-Text
10550 690 2007 12 PDF Available
Title
Upregulation of bone cell differentiation through immobilization within a synthetic extracellular matrix
Abstract

There is a need for new therapeutic strategies to treat bone defects caused by trauma, disease or tissue loss. Injectable systems for cell transplantation have the advantage of allowing the use of minimally invasive surgical procedures, and thus for less discomfort to patients. In the present study, it is hypothesized that Arg–Gly–Asp (RGD)-coupled in a binary (low and high molecular weight) injectable alginate composition is able to influence bone cell differentiation in a three-dimensional (3D) structure. Viability, metabolic activity, cytoskeleton organization, ultrastructure and differentiation (alkaline phosphatase (ALP), von Kossa, alizarin red stainings and osteocalcin quantification) of immobilized cells were assessed. Cells within RGD-modified alginate microspheres were able to establish more interactions with the synthetic extracellular matrix as visualized by confocal laser scanning microscope and transmission electron microscopy imaging, and presented a much higher level of differentiation (more intense ALP and mineralization stainings and higher levels of osteocalcin secretion) when compared to cells immobilized within unmodified alginate microspheres. These findings demonstrate that peptides covalently coupled to alginate were efficient in influencing cell behavior within this 3D system, and may provide adequate preparation of osteoblasts for cell transplantation.

Keywords
Osteoblasts; Cell encapsulation; Alginate; RGD peptide; ECM (extracellular matrix); Bone regeneration
First Page Preview
Upregulation of bone cell differentiation through immobilization within a synthetic extracellular matrix
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 25, September 2007, Pages 3644–3655
Authors
, , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering