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Proliferation and differentiation of neural stem cells on lysine–alanine sequential polymer substrates

Paper ID Volume ID Publish Year Pages File Format Full-Text
10583 692 2006 10 PDF Available
Title
Proliferation and differentiation of neural stem cells on lysine–alanine sequential polymer substrates
Abstract

The purpose of this study was to explore the phenotypic potential of embryonic rat cerebral cortical stem cells by inducing differentiation on lysine–alanine sequential (LAS) polymer substrates at neurosphere level. LAS polymer is a heterologous polymer of lysine and alanine and has been demonstrated to enhance axon growth of neurons in a serum-free medium in vitro. It was found that very few cells migrated outside of the neurospheres but extremely long processes extended from differentiated cells could form a network between remote neurospheres when cells were cultured on LAS substrates at a low density of 120 neurospheres/cm2 in the serum-free medium. On the contrary, when the neurosphere density was increased to 360 neurospheres/cm2, many neurosphere-forming cells migrated out from their original aggregate and exhibited short processes morphology. Furthermore, when serum was added to the culture system, the neurosphere-forming cells could be induced into an extensive cellular substratum of protoplasmic cells upon which process-bearing cells spread. Clearly, neurospheres could exhibit different behaviors on LAS substrates according to the complex environmental conditions. Here, we proposed that neurospheres would change their social communication and adopt different strategies to communicate with other neurospheres when they detected each other's presence. Therefore, the mediation of cell behavior on LAS substrates by communication between neurospheres should be taken into account.

Keywords
Lysine–alanine sequential (LAS) polymer; Neural stem cells
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Proliferation and differentiation of neural stem cells on lysine–alanine sequential polymer substrates
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 18, June 2006, Pages 3441–3450
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us