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Construction of epidermal growth factor fusion protein with cell adhesive activity

Paper ID Volume ID Publish Year Pages File Format Full-Text
10584 692 2006 8 PDF Available
Title
Construction of epidermal growth factor fusion protein with cell adhesive activity
Abstract

A novel protein for controlling cellular functions was constructed by combining functional units of various proteins. The Arg–Gly–Asp (RGD) sequence functioning as a cell adhesive function, an epidermal growth factor (EGF) as a cell growth function, and a hydrophobic sequence (E12) as an efficient assembling function, were combined and incorporated into one molecule. The fusion protein, designated ERE–EGF, was produced in Escherichia coli and purified with affinity chromatography using a His–tag. The ERE–EGF coated on an unmodified hydrophobic surface of a cell-culture plate (through the hydrophobic E12 moiety) retained both cell adhesive activity (through the RGD sequence) and cell growth activity (through the EGF moiety).

Keywords
Epidermal growth factor; RGD; Hydrophobic structure; Fusion protein; Cell adhesion
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 18, June 2006, Pages 3451–3458
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us