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Fabrication of a phospholipid membrane-mimetic film on the luminal surface of an ePTFE vascular graft

Paper ID Volume ID Publish Year Pages File Format Full-Text
10587 692 2006 9 PDF Available
Title
Fabrication of a phospholipid membrane-mimetic film on the luminal surface of an ePTFE vascular graft
Abstract

A stabilized, membrane-mimetic film was produced on the luminal surface of an ePTFE vascular graft by in situ photopolymerization of an acrylate functonalized phospholipid using a fiber optic diffusing probe. The phospholipid monomer was synthesized, prepared as unilamellar vesicles, and fused onto close-packed octadecyl chains that were components of an amphiphilic terpolymer anchored onto the polyelectrolyte multilayer (PEM) by electrostatic interactions. Scanning electron microscopy (SEM) confirmed that gelatin impregnation of the graft followed by the subsequent biomimetic film coating filled in the fibril and node structure of the luminal surface of the ePTFE graft and was smooth. The lipid film displayed an initial advancing contact angle of 44°, which increased to 55° after being subjected to a wall shear rate of 500 s−1 for 24 h at 37 °C in phosphate buffered saline (PBS). Fourier transform (FT-IR) spectroscopy was used to characterize the stages of biomimetic film assembly and confirmed the stability of the film under shear flow conditions. In vivo assessment using a baboon femoral arteriovenous shunt model demonstrated minimal platelet and fibrinogen deposition over a 1-h blood-contacting period. The results of this study confirm the versatility of a biomimetic film coating system by successfully transferring the methodology previously developed for planar substrates to the luminal surface of an ePTFE vascular graft.

Keywords
Vascular graft; Biomimetics; Membrane-mimetic; Blood-contacting materials; Thrombogenicity
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Fabrication of a phospholipid membrane-mimetic film on the luminal surface of an ePTFE vascular graft
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 18, June 2006, Pages 3473–3481
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us