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Augmentation of skeletal tissue formation in impaction bone grafting using vaterite microsphere biocomposites

Paper ID Volume ID Publish Year Pages File Format Full-Text
10600 693 2009 10 PDF Available
Title
Augmentation of skeletal tissue formation in impaction bone grafting using vaterite microsphere biocomposites
Abstract

The development of particulate bone void fillers with added biological function to augment skeletal tissue formation will lead to improved efficacy in bone replacement surgery. We demonstrate the potential for vaterite microsphere biocomposites to augment bone matrix formation within an in vivo model for impaction bone grafting seeded with human bone marrow stromal cells. In vitro tests demonstrate the significance of vaterite microspheres in the activation and promotion of 3D skeletal tissue formation. Further in vitro experiments using functionalized microspheres with surface integrated RGD peptide activate co-cultured skeletal populations in pellets and promote secretion of extracellular matrix collagens and human osteocalcin. Specific temporal release of entrapped RNase A was successfully demonstrated using these specialized microspheres with integrated magnetic beads, which physically disrupted the inorganic macrostructure. These studies demonstrate that bio-inspired calcium carbonate microspheres augment in vivo bone formation in impaction bone grafting. Such microspheres with added biological functionality offer innovative therapeutic approaches to activate skeletal populations and enhance bone formation with reparative implications for hard tissues.

Keywords
Human osteoprogenitor; Calcium carbonate; Osteogenesis; Biomimetic material; Mineralization; Bone tissue engineering
First Page Preview
Augmentation of skeletal tissue formation in impaction bone grafting using vaterite microsphere biocomposites
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 10, April 2009, Pages 1918–1927
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering