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Practical recombinant hybrid mussel bioadhesive fp-151

Paper ID Volume ID Publish Year Pages File Format Full-Text
10639 695 2007 9 PDF Available
Title
Practical recombinant hybrid mussel bioadhesive fp-151
Abstract

Mussel adhesive proteins (MAPs) have received increased attention as potential environmentally friendly adhesives under aqueous conditions and in medicine. However, attempts to produce functional recombinant MAPs (mainly foot protein type 1, fp-1) by several expression systems have failed. Even though we previously reported a functional expression of recombinant foot protein type 5 (fp-5) with significant adhesive ability in Escherichia coli, its practical use was limited by several problems such as low production yield, low purification yield, and high levels of post-purification insolubility. Here, to overcome these limitations, we designed and constructed the novel type of hybrid mussel bioadhesive fp-151, a fusion protein comprising six fp-1 decapeptide repeats at each fp-5 terminus. Using micro- and bulk-scale characterization and mammalian cell-adhesion analyses, we demonstrate that fp-151 has the potential to be a practical bioadhesive with strong adhesive ability, a simple purification process (∼1 g-purified protein per 1 l-pilot-scale fed-batch bioreactor culture), proper manipulation properties (∼330 g/l solubility), and high biocompatibility.

Keywords
Mussel adhesive protein; Hybrid fp-151; Bioadhesive; Fusion protein; Escherichia coli; Cell adhesive
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 24, August 2007, Pages 3560–3568
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us